We compared the hydroxyl radicalscavenging activity of 25 antioxidant sam ples prepared in ethanol solution with samples prepared after removing the ethanol residue. Read kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a fentontype reaction system, analytica chimica acta on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Deoxyribose degradation assay oh free radical scavenging activity of samples was measured with the procedure described by burits et al. They were also effective oh radical scavenging agents in the assay for non. Phenolic constituents of eucalyptus camaldulensis dehnh, with potential antioxidant and cytotoxic activities. The % inhibition of hydroxyl radical was calculated and calculation.
This article is downloadable online in pdf format at introduction. Simultaneous detection of pro and antioxidative effects in the variants of the deoxyribose degradation assay. The deoxyribose degradation assay was used to evaluate the hydroxyl radical scavenging activity 42. This degradation was inhibited by catalase and desferrioxamine but not by sod not shown. The origin of purine and pyrimidine deoxyribose in. Deoxyribose assay is used to determine the hydroxyl radical scavenging activity in an aqueous medium. Formation of thiobarbituricacidreactive substance from deoxyribose in the presence of iron salts.
Antioxidant activity of nigella sativa essential oil. The deoxyribose degradation assay is widely used to evaluate the hydroxyl oh radicalscavenging abil ity of food or medicines. Antioxidant activities of three rubus species growing in. The deoxyribose degradation assay is widely used to evaluate the hydroxyl oh radicalscavenging ability of food or medicines. A comparative study on ripe and unripe eggplant solanum. Changes in ph, weight of dry mycelia and dnadepolymerase activity during growth. Deoxyribose is enzymically formed in living cells by reduction of ribonucleoside di or triphosphate.
Request pdf kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a fentontype reaction. We compared the hydroxyl radicalscavenging activity of 25 antioxidant samples prepared in ethanol solution with samples prepared after removing the ethanol residue. In vitro antioxidant activity of vetiveria zizanioides. Degradation of nucleic acids and their related compounds. The different fractions inhibited lipid peroxidation, repressed the production of nitric oxide radicals, and prevented deoxyribose degradation.
Edta showed that the antioxidant activity is independent. Importance of deoxyribose degradation assay for evaluating. Deoxyribose degradation was measured as tbars and the percentage inhibition was calculated 21. Deoxyribose a nucleic acid constituent see illustration of all animal, microbial, and plant cells.
Catechin, eriodictyol and taxifolin vladimir chobot 1, franz hadacek 2, gert bachmann 1, wolfram weckwerth 1 and. Modification of the deoxyribose test to detect strong iron binding. Kinetic deoxyribose degradation assay and its application. In the deoxyribose degradation assay the 80% methanol, acetone and methanol extracts of r. Deoxyribose degradation was measured as tbars by the method of ohkawa ohkawa et. Solvent effects and improvements in the deoxyribose. Pro and antioxidant activity of three selected flavan. Iron ions play a central role in oh radicals formation and induction of oxidative stress in. Deoxyribose article about deoxyribose by the free dictionary. Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using the frap, bps, and deoxyribose methods. Methods of measuring the antioxidant activity and antioxidant content in coffee. Is there any easy method for the estimation of hydroxyl radical. Prodxidant iron and copper, with ferroxidase and xanthine.
Phenolic constituents of eucalyptus camaldulensis dehnh. Deoxyribose deoxyribose deoxyribose deoxyribose 8 of 9 answer key making a model of dna 5 construct the right side of your dna model by putting together in sequence a cytosine, thymine, guanine and adenine nucleotide. Invitro antioxidant and free radical scavenging activity. Simultaneousdetectionofproandantioxidativeeffectsinthe. Protection of deoxyribose and dna from degradation by. The origin of purine and pyrimidine deoxyribose in escherichia coli systems marilyn r. The antimutagenic activity of the leaf fractions was analyzed against 4nitroophenylenediamine, sodium azide and 2aminofluorene mutagens in two test strains ta98 and ta100 of salmonella typhimurium. Consequently, scoring was performed only after 16 h. Kinetic deoxyribose degradation assay and its application in. Labeling of glucose, ribose, and deoxyribose by l and 2c4glycine in regenerating rat liver waltw w. Evaluation of antioxidant and free radical scavenging activity of. The experiment was conducted using pre and coincubation modes. Vladimir chobot department of chemical ecology and ecosystem research, faculty of life sciences, university of vienna, althanstrasse 14, a1090 vienna, austria. Phytochemical studies, antioxidant activity and protective.
Ohradical scavengers such as formate, thiourea and mannitol. The degradation of 2 deoxyribose to thiobarbituric acidreactive material was investigated with two hydroxylradicalgenerating systems. These antioxidant and free radical scavenging activities of sesamol. Deoxyribose degradation strongly depended on the diffusion of air oxygen into the liquid. In each case the thiobarbituric acidreactive material can be accounted for by malondialdehyde, as measured by an h. Antioxidant and free radical scavenging potential have been reported to be rich in the fresh leaf than dry leaf 5. The radical scavenging activity of the polysaccharides against hydroxyl radicals was measured using the method of ohkawa et al. Ribose of rna was isolated and purified 11 and degraded with. Simultaneous detection of pro and antioxidative effects. Labeling of glucose, ribose, and deoxyribose by l and 2.
Inhibitory effects of the polysaccharides on deoxyribose degradation were determined by measuring the competition between deoxyribose and the. The absence of hydrogen peroxide and ascorbic acid prevented the initialization of fenton reaction in both variants with and without edta when the scoring was performed within one hour. One unit of xanthine oxidase converts 1,umol of xanthine to uric acid per minute. In deoxyribose degradation assay, the ive, ivea and protocatechuic acid also demonstrated antioxidant activity with ic50 of approximately 2000, 1400 and 600. Mitomycin cinduced deoxyribose degradation inhibited by. By degradation of the ribose and subsequent determination. The low method determination limit makes the deoxyribose degradation reaction potentially useful as a new, highly sensitive and cost effective assay for iron quantification. Inhibition by the enzyme superoxide dismutase implies that the reduction of the ferricedta complex for participation in the fenton reaction is. Its name indicates that it is a deoxy sugar, meaning that it is derived from the sugar ribose by loss of an oxygen atom.
Effect of organic cosolvents in the evaluation of the. Antioxidant activity of nigella sativa essential oil m. Table 3 mitomycin cinduced deoxyribose degradation in the presence of xanthine oxidase under anaerobic condi tions. In vitro antioxidant and free radical scavenging activities of. Synthesis of hydroxyl radical scavengers from benzalacetone and its derivatives. Deoxyribose definition of deoxyribose by medical dictionary. Furthermore, oh radicals can also enhance dna damage, via attack on its phosphate bonds.
Chemical composition and antioxidant properties of juniper. Moreover, kyna showed no antioxidant effects in the fecl3ascorbic acid deoxyribose degradation assay variant and only negligible activities. One of the major drawbacks of the assay, however, is that only water soluble compounds can be tested for their radicalscavenging activity. The results were subjected to linear regression and ic 50 value was calculated 8.
Free radical reactions and antioxidant activities of sesamol. Degradation of deoxyribose and benzoate and the hydroxylation of benzoate are substantially inhibited by superoxide dismutase and. A similar order of reactivity was found in the case of 2 deoxyribose. Biocatalysis design of an in vitro biocatalytic cascade. Juglone showed pronounced antioxidative effects by decreasing the deoxyribose degradation in both systems, either with or without addition of edta. The blank negative control contained the full reaction mixture without 2deoxy d ribose 0% mda. Deoxyribose degradation was determined as described in literature. The four deoxyribose nucleotides, containing adenine, guanine, cytosine, and thymine, are the major constituents of the.
Valerian is widely used as a traditional medicine to improve the quality of sleep. Hydroxyl radical ohx scavenging activity by 2 deoxyribose 2dr degradation assaythe hydroxyl radical ohx scavenging activity of gac, carrots and tomatoes was assessed using the 2 deoxyribose 2dr degradation assay genaromattos, 2009. Degradation of nucleic acids and their related compounds by microbial enzymes. The various antioxidant activities were compared with suitable antioxidants such as butyl hydroxy toluene. Ms analysis of the essential oil obtained from six different samples of nigella sativa seeds and from a commercial fixed oil showed that the qualitative composition of the volatile. In vitro chemical measures of antioxidant activity employed are dpph radical scavenging, reducing properties, iron chelation, prevention of both deoxyribose degradation and lipid peroxidation by the seed and flesh of both ripe and unripe eggplant. The hydroxyl radical scavenging activity of the extract is reported as % inhibition of deoxyribose degradation is. Hydroxylradicalinduced ironcatalysed degradation of 2. Pro and antioxidant activity of three selected flavan type flavonoids. Deoxyribose, or more precisely 2 deoxyribose, is a monosaccharide with idealized formula h. Invitro antioxidant and free radical scavenging activity of bauhinia variegata. In vitro antioxidant activity of valeriana officinalis.
Evaluation of antioxidant and free radical scavenging. Kinetic analysis of some chalcones and synthetic chalcone. In this method, 100ml of 28mm, 2deoxydribose in phosphate buffer ph 7. The hydroxyl radical scavenging activity at different concentrations of extract 10 to 250. Lipid peroxidation and deoxyribose degradation linolenic acid miceues, and deoxyribose 14 were used as sub. Antioxidant activity of the essential oils of different. The antimutagenic activity of the leaf fractions was analyzed against 4nitroophenylenediamine, sodium azide and 2amino. Several dera homologs displayed high activity in the retro.
Get a printable copy pdf file of the complete article 450k, or click on a page. Evaluation of in vitro antioxidant and antibacterial. Dpph radical scavenging activity the antioxidant activity of the extracts, based on the. Antioxidant and antimutagenic activities of different. View the article pdf and any associated supplements and figures for a period of 48 hours. Invitro antimicrobial and antioxidant activities of. The low method determination limit makes the deoxyribose degradation reaction. Synthesis of hydroxyl radical scavengers 62 benzalacetone has a conjugated system and is expected to be easily oxidized. Iron ions play a central role in oh radicals formation and induction of oxidative stress in living organisms. Protocatecheuic acid underlies the antioxidant activity. The deoxyribose degradation assay has been widely used to evaluate the hydroxyl oh radicalscavenging ability of food, nutrients, and. The antioxidant activity aa is measured by various chemical and physicochemical methods. The 2deoxyribose degradation assay 2dr assay is an in vitro method broadly used for evaluating the scavenging activity against the hydroxyl radical ho.
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